Investigating the Genetic Background of Proteoglycan-Induced Arthritis and Proteoglycan- Induced Ankylosing Spondylitis
نویسنده
چکیده
Introduction: The MHC (H-2d) and non-MHC genetic background make the BALB/c strain highly susceptible to inflammatory arthritis and spondylitis. Although different BALB/c colonies develop proteoglycan (PG)-induced arthritis (PGIA) and spondylitis (PGIS) in response to immunization with human cartilage PG, they show significant differences in disease penetrance, despite being maintained by the same vendor either at the same or different location. Methods: BALB/c female mice (24-26 weeks old after 4 weeks acclimatization), were immunized with a suboptimal dose of cartilage PG to explore even minute differences among 11 subcolonies purchased from five different vendors. Serum cytokines and (auto)antibodies, in vitro measured T cell responses were correlated with arthritis (and spondylitis) phenotypic scores. cDNA microarrays were also performed using spleen cells of naïve and immunized BALB/cJ and BALB/cByJ mice (both colonies from The Jackson Laboratories) which represent the two major BALB/c sublines.
منابع مشابه
Investigating the Genetic Background of Proteoglycan- Induced Arthritis and Proteoglycan-Induced Ankylosing Spondylitis
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